Setting standards for polymer click chemistry

Angew Chem. Int. Ed. 2011, 50, 60-62. DOI: 10.1002/anie.201003707
A group of polymer click chemistry leaders have tried to set the standards for the field - and for the terminology "polymer click chemistry" to be meaningful. 

To add a few other points. Polymer chemistry has been using the most efficient reactions for a long time, before the click chemistry philosophy. Core values of click chemistry need to be defended - many published claims are not in the spirit of click chemistry.Click chemistry is not equal to "efficient" or "successful". Click chemistry should meet the set high standards and remain fluid as a philosphy. Exceptions should be allowed in extremely challenging complex systems, such as "spying on" the activities inside a live cell.    
                                                        

Cholesterol & protein studied by Click chemistry

Chemical Communications Received 31st October 2010, Accepted 21st December 2010 DOI: 10.1039/c0cc04710d

Tate and coworkers from Imperial College London reported the bioorthogonal click chemistry of a fluorescent alkyne probe with azido-modified post-translationally cholesterylated proteins - in living cells. The design is shown in the drawing. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation. This method, in comparison with the current radiolabeling, offers safety, speed, sensitivity, and flexibility. It may allow the observation of protein cholesterylation over space and time using fluorescence microscopy.

In more complex and challenging environment, click chemistry has found application and success, only due to its extremely high efficiency and orthogonality.