Click chemistry in protein fatty acylation and prenylation

Nature Chemical Biology, Published online June 17, 2010 

The above and many other click chemistry reagents were chemically synthesized, metabolically or enzymatically introduced into target proteins to effect extremely sensitive detection, by western blotting or in-gel fluorescence. The applications of these probes are: identifying new lipid-modified proteins, analyzing the lipid content of cellular proteins, imaging lipid-modified proteins in cells, and tools for protein labeling in vitro. 

The success lies on the qualities of click chemistry: reaction efficiency, functional group and reaction condition tolerance,  small size of azido and alkenyl groups, etc.   

         

Click chemistry for conjugated molecular wire

J. Am. Chem. Soc., Article ASAP, DOI: 10.1021/ja103239b
Publication Date (Web): June 15, 2010

The article demonstrates a beautiful example of click chemistry for the synthesis of long (>4nm) pi-conjugated wires (all-aromatic backbone structure) bonded to metal electrodes.

Elegantly designed, nicely executed, fully characterized, and convincingly presented.

Aromatic and rigid triazole structure from click chemistry is explored to deliver the unique property for the applications in materials science and nanotechnology.  

The measurement confirms the length-dependent conductance and mechanism transition.