Click chemistry in protein fatty acylation and prenylation

Nature Chemical Biology, Published online June 17, 2010 

The above and many other click chemistry reagents were chemically synthesized, metabolically or enzymatically introduced into target proteins to effect extremely sensitive detection, by western blotting or in-gel fluorescence. The applications of these probes are: identifying new lipid-modified proteins, analyzing the lipid content of cellular proteins, imaging lipid-modified proteins in cells, and tools for protein labeling in vitro. 

The success lies on the qualities of click chemistry: reaction efficiency, functional group and reaction condition tolerance,  small size of azido and alkenyl groups, etc.